AmpTec Amplification Technologies

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Product Catalogue | Application Notes | Publications | Reference Users

Product Catalogue with C&E

ExpressArt® Kit Versions

Custom Service for synthetic functional mRNAs
For more information see our recent publication Quabius & Krupp, 2014
Synthetic mRNAs for manipulating cellular phenotypes: an overview. New Biotechnol. (2014), http://dx.doi.org/10.1016/j.nbt.2014.04.008

Applications
1) Stem cell generation (iPCs) Reprogramming of somatic cells with a defined mix of synthetic highly modified mRNAs: m5C and/or pseudo-U replace standard C or U (100% or customer-specified levels) to prevent innate immune response
2) Vaccination with high specificity and high efficiency is achieved via ex vivo transformation of isolated autologous dendritic cells with synthetic mRNA or mRNA mixtures
3) Cellular expression patterns can be changed at will, without permanent genomic changes
Please inquire for a custom-made mRNA product

Custom Service for synthetic RNAs, dsDNAs & plasmid DNAs (w/o Ecoli DNA)
These nucleic acid products are available at two levels:
1) research-grade
IVT-RNAs and dsDNA products, manufactured in compliance with regulations of ISO13485
2) NEW OPTION GMP-grade
IVT-RNAs, manufactured in compliance with regulations of FDA 21 CFR Part 820
(cGMP manufacturing) for more information click here

Applications
1) In Vitro Diagnostics (e.g. viral RNA) & detection of rare gene mutants
2) Companion Diagnostics: a rapidly increasing application field
Increase reliability of diagnostic qPCR assays by continuous performance monitoring
Develop new qPCR assays with a free choice of any target sequence
Include high-level IVT-RNA GMP products as components of your diagnostic kits
Please inquire for a custom-made RNA or DNA product

Custom-made long RNAs with 5'-Modifications
Possibilities include, but are not limited to 5'-Terminal Biotin or Thiol for immobilisation, e.g. for long RNA aptamers, 5'-Terminal Dyes for detection/visualization, no interference with (biological) RNA functions.
Please inquire for a custom-made 5'-modified product
C&E Micro Add-On dsDNA Synthesis
Conversion of 1-round amplified RNA (depleted of rRNA sequences) to dsDNA

C&E Nano Add-On dsDNA Synthesis
Conversion of 2-rounds amplified RNA (depleted of rRNA sequences) to dsDNA

ExpressArt® C&E mRNA amplification kits
» "Extended Manual" with general technology information
Catalogue No. Protocol (PDF) MSDS (PDF)
C&E Micro Kit 7199-A30 61 KB MSDS
C&E Nano Kit 7299-A15 92 KB MSDS
C&E Pico Kit 7399-A15 114 KB MSDS

Special I: ExpressArt® TRinucleotide mRNA Amplification Kits for degraded RNA & FFPE RNA suitable for whole transcript (Exon or Gene ST) arrays
» "Extended FFPE Manual" with general technology information &
» "Extended ST Array Manual" with general technology information

Catalogue No.

Manual (PDF)

MSDS (PDF)
C&E TRinucleotide Micro Kit 6199-A30 63 KB MSDS
C&E TRinucleotide Nano Kit 6299-A15 92 KB MSDS
C&E TRinucleotide Pico Kit 6399-A15 116 KB MSDS

Special II: ExpressArt® Bacterial mRNA Amplification Kits
» "Extended Bacterial Manual" with general technology information

Catalogue No.

Protocol (PDF)

MSDS (PDF)
C&E Bacterial Micro Kit 5199-A30 61 KB MSDS
C&E Bacterial Nano Kit 5299-A15 92 KB MSDS

ExpressArt® Add-On Modules

Catalogue No.

Manual (PDF)

MSDS (PDF)
AminoAllyl Add-On Module AA30
Generation of AminoAllyl modified RNAs for dye coupling and RNA fragmentation, NHS-activated dyes are not included
2000-AA30 132 KB MSDS
AminoAllylAdd-On Module AA15
Generation of AminoAllyl modified RNAs for dye coupling and RNA fragmentation, NHS-activated dyes are not included
2000-AA15 132 KB MSDS

cDNA synthesis kits

Catalogue No.

Manual (PDF)

MSDS (PDF)
TR cDNA synthesis kit
Generate cDNAs with the unique ExpressArt TRinucleotide priming technology: full-length plus selectivity against rRNA sequences
8994-A30 48 KB MSDS
Bacterial H-TR cDNA synthesis kit
Special primer version for extreme selectivity against rRNA sequences
8004-A30 48 KB MSDS
Eukaryotic H-TR cDNA synthesis kit
Special primer version for extreme selectivity against rRNA sequences
8114-A30 48 KB MSDS

RNA/DNA Isolation with ExpressArt® Kits and Reagents

Catalogue No.

Manual (PDF)

MSDS (PDF)
NucleoGuard
Universal nuclease and RNase inhibitor for improved RNA quality
8998-M50 105 KB MSDS
Pico RNA Care
Carrier compounds for small samples (< 10ng RNA) N-Carrier (RNA) and P-Carrier (for EtOH precipitations)
8999-A100 80 KB MSDS
N-Carrier now separately available
8997-A100 74 KB MSDS
FFPE RNAready
FFPE RNA isolation kit includes NG and decrosslinking reagents (patent pending)
9000-A100 43 KB MSDS
FFPE Clear RNAready
FFPE RNA isolation kit eliminates the paraffin removal step and reduces processing time
9009-A100 36,2 KB MSDS
LCM RNAready
LCM RNA isolation kit includes NG (RNase inhibitor) and N-Carrier (inert RNA)
9001-A100 47 KB MSDS
LBR RNAready
Total RNA isolation kit: Bacteria & Solid tissues adapted for mechanical lysis with beads
9005-A100 45 KB MSDS
RNAready Add-On
Spin column RNA isolation kit as Add-On for GTC-phenol-chloroform extraction (e.g. Trizol)
9007-A100 45 KB MSDS
RNAready
RNA isolation kit
9002-A100 28 KB MSDS
RNA Clean-up
RNA Clean RNAready
9003-A100 42 KB MSDS
DNA Clean-up
DNA Clean DNAready
9004-A100 26 KB MSDS