AmpTec Amplification Technologies

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ExpressArt® Specials

ExpressArt®: The future of mRNA amplification
APPLICATIONS WITH SPECIAL ADVANTAGES

LASER MICRODISSECTION
  • Very small samples ~ cells or 100 picogram total RNA.
  • No need for calibrated amounts in sample series: directly compare small areas with a few cells and large areas with thousands of cells.
  • Option to perform 3 amplification rounds.
  • Archival Templates: in vitro transcription test reaction, before using labelled NTPs.
  • No need for optimal RNA quality, optimized "TR technology" for recovery of all mRNA sequences - independent of poly-A.

BACTERIAL mRNA AMPLIFICATION
  • No need to remove rRNAs: "TR technology" enables selection against rRNAs (<2% in amplified RNAs) and priming near the 3'- ends of mRNAs - independent from a universal sequence (like poly-A).
  • Universal selection against rRNAs (intact and degraded) based on structure, not based on sequence.
  • Option to perform 2 amplification rounds.
  • Very small samples ~ 50,000 bacterial cells or 1 nanogram total RNA.
  • Very flexible microarray design is available - customised for your species!

PARAFFIN (FFPE) SAMPLES
  • The only FFPE kit with low amounts of rRNA sequences (<2%) in amplified RNAs!
  • Optimized "TR technology" for recovery of all mRNA sequences - by priming near the 3'-ends of all mRNA fragments, independent of poly(A).
  • Flexible with large and small samples, with 1 or 2 amplification rounds.

WHOLE TRANSCRIPT ARRAYS: EXON AND GENE ST ARRAYS
  • Use degraded RNA samples and paraffin (FFPE) samples.
  • Very simple procedure: the only kit which does not require rRNA removal, combined with low amounts of rRNA sequences (<2%) in amplified RNAs! therefore, without compromise in detection sensitivity!
  • Optimized "TR technology" for recovery of whole transcript mRNA sequences - by priming near the 3'-ends of all mRNA fragments & by additional internal priming at stop sites of reverse transcriptase.
  • Flexible with large and small samples, with 1 or 2 amplification rounds.

For more informatins of ExpressArt® Specials click here.